Human Gene CYTH4 (uc003arf.3) Description and Page Index
  Description: Homo sapiens cytohesin 4 (CYTH4), mRNA.
RefSeq Summary (NM_013385): This gene encodes a member of the PSCD family of proteins, which have an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family function as GEPs for ADP-ribosylation factors (ARFs), which are guanine nucleotide-binding proteins involved in vesicular trafficking pathways. This protein exhibits GEP activity in vitro with ARF1 and ARF5, but is inactive with ARF6. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2015].
Transcript (Including UTRs)
   Position: hg19 chr22:37,678,495-37,711,389 Size: 32,895 Total Exon Count: 13 Strand: +
Coding Region
   Position: hg19 chr22:37,678,611-37,709,552 Size: 30,942 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr22:37,678,495-37,711,389)mRNA (may differ from genome)Protein (394 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Cytohesin-4; AltName: Full=PH, SEC7 and coiled-coil domain-containing protein 4;
FUNCTION: Promotes guanine-nucleotide exchange on ARF1 and ARF5. Promotes the activation of ARF through replacement of GDP with GTP.
TISSUE SPECIFICITY: Expressed predominantly in peripheral blood leukocytes.
SIMILARITY: Contains 1 PH domain.
SIMILARITY: Contains 1 SEC7 domain.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): CYTH4
CDC HuGE Published Literature: CYTH4

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 126.54 RPKM in Whole Blood
Total median expression: 326.46 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -43.60116-0.376 Picture PostScript Text
3' UTR -787.841837-0.429 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology
IPR000904 - Sec7
IPR023394 - SEC7_alpha_orthog

Pfam Domains:
PF00169 - PH domain
PF01369 - Sec7 domain

SCOP Domains:
48425 - Sec7 domain
50729 - PH domain-like

ModBase Predicted Comparative 3D Structure on Q9UIA0
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005086 ARF guanyl-nucleotide exchange factor activity
GO:0008289 lipid binding

Biological Process:
GO:0032012 regulation of ARF protein signal transduction

Cellular Component:
GO:0000139 Golgi membrane
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane

-  Descriptions from all associated GenBank mRNAs
  AF125349 - Homo sapiens guanine nucleotide exchange factor cytohesin-4p mRNA, complete cds.
JD309705 - Sequence 290729 from Patent EP1572962.
AF075458 - Homo sapiens cytohesin-4 (CYT4) mRNA, complete cds.
JD375040 - Sequence 356064 from Patent EP1572962.
AK304453 - Homo sapiens cDNA FLJ54256 complete cds, highly similar to Cytohesin-4.
BC017780 - Homo sapiens cytohesin 4, mRNA (cDNA clone IMAGE:4691010), complete cds.
BC033693 - Homo sapiens cytohesin 4, mRNA (cDNA clone IMAGE:5213325), with apparent retained intron.
BC041161 - Homo sapiens cytohesin 4, mRNA (cDNA clone MGC:48780 IMAGE:5745783), complete cds.
AK024428 - Homo sapiens mRNA for FLJ00017 protein, partial cds.
CR456551 - Homo sapiens PSCD4 full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.PSCD4).
AK315052 - Homo sapiens cDNA, FLJ96001, highly similar to Homo sapiens pleckstrin homology, Sec7 and coiled-coil domains 4 (PSCD4), mRNA.
JD180046 - Sequence 161070 from Patent EP1572962.
KJ902452 - Synthetic construct Homo sapiens clone ccsbBroadEn_11846 CYTH4 gene, encodes complete protein.
AB463181 - Synthetic construct DNA, clone: pF1KF0017, Homo sapiens PSCD4 gene for pleckstrin homology, Sec7 and coiled-coil domains 4, without stop codon, in Flexi system.
CU013439 - Homo sapiens PSCD4, mRNA (cDNA clone IMAGE:100000408), complete cds, without stop codon, in Gateway system.
CU013151 - Homo sapiens PSCD4, mRNA (cDNA clone IMAGE:100000504), complete cds, with stop codon, in Gateway system.
AK002145 - Homo sapiens cDNA FLJ11283 fis, clone PLACE1009524, moderately similar to ARF NUCLEOTIDE-BINDING SITE OPENER.
JD545683 - Sequence 526707 from Patent EP1572962.
JD099844 - Sequence 80868 from Patent EP1572962.
JD338505 - Sequence 319529 from Patent EP1572962.
JD463489 - Sequence 444513 from Patent EP1572962.
JD179637 - Sequence 160661 from Patent EP1572962.
JD073943 - Sequence 54967 from Patent EP1572962.
JD469208 - Sequence 450232 from Patent EP1572962.
JD102957 - Sequence 83981 from Patent EP1572962.
JD063312 - Sequence 44336 from Patent EP1572962.
JD272421 - Sequence 253445 from Patent EP1572962.
JD479186 - Sequence 460210 from Patent EP1572962.
JD058118 - Sequence 39142 from Patent EP1572962.
JD237583 - Sequence 218607 from Patent EP1572962.
JD311527 - Sequence 292551 from Patent EP1572962.
JD353292 - Sequence 334316 from Patent EP1572962.
JD050227 - Sequence 31251 from Patent EP1572962.
JD339157 - Sequence 320181 from Patent EP1572962.
JD054785 - Sequence 35809 from Patent EP1572962.
JD252424 - Sequence 233448 from Patent EP1572962.
JD117685 - Sequence 98709 from Patent EP1572962.
JD330489 - Sequence 311513 from Patent EP1572962.
JD302186 - Sequence 283210 from Patent EP1572962.
JD063938 - Sequence 44962 from Patent EP1572962.
JD333136 - Sequence 314160 from Patent EP1572962.
JD188708 - Sequence 169732 from Patent EP1572962.
JD170508 - Sequence 151532 from Patent EP1572962.
JD155519 - Sequence 136543 from Patent EP1572962.
JD260669 - Sequence 241693 from Patent EP1572962.
JD316393 - Sequence 297417 from Patent EP1572962.
JD358148 - Sequence 339172 from Patent EP1572962.
JD487838 - Sequence 468862 from Patent EP1572962.
JD193375 - Sequence 174399 from Patent EP1572962.
JD133042 - Sequence 114066 from Patent EP1572962.
JD365625 - Sequence 346649 from Patent EP1572962.
JD262367 - Sequence 243391 from Patent EP1572962.
JD195045 - Sequence 176069 from Patent EP1572962.
JD492170 - Sequence 473194 from Patent EP1572962.
JD294930 - Sequence 275954 from Patent EP1572962.
JD384370 - Sequence 365394 from Patent EP1572962.
JD496421 - Sequence 477445 from Patent EP1572962.
JD393882 - Sequence 374906 from Patent EP1572962.
JD137370 - Sequence 118394 from Patent EP1572962.
JD145062 - Sequence 126086 from Patent EP1572962.
JD355927 - Sequence 336951 from Patent EP1572962.
JD447743 - Sequence 428767 from Patent EP1572962.
JD113312 - Sequence 94336 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_arapPathway - ADP-Ribosylation Factor

Reactome (by CSHL, EBI, and GO)

Protein Q9UIA0 (Reactome details) participates in the following event(s):

R-HSA-8847880 CYTH proteins bind ARF1:GTP
R-HSA-8847883 CYTH proteins stimulate ARF1 GTPase activity
R-HSA-6811438 Intra-Golgi traffic
R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic
R-HSA-199991 Membrane Trafficking
R-HSA-5653656 Vesicle-mediated transport

-  Other Names for This Gene
  Alternate Gene Symbols: CYH4_HUMAN, CYT4, NM_013385, NP_037517, PSCD4, Q5R3F9, Q9UGT6, Q9UIA0
UCSC ID: uc003arf.3
RefSeq Accession: NM_013385
Protein: Q9UIA0 (aka CYH4_HUMAN)
CCDS: CCDS13946.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_013385.3
exon count: 13CDS single in 3' UTR: no RNA size: 3138
ORF size: 1185CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2570.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.