Human Gene PTGES3 (uc001slu.4) Description and Page Index
  Description: Homo sapiens prostaglandin E synthase 3 (cytosolic) (PTGES3), mRNA.
RefSeq Summary (NM_006601): This gene encodes an enzyme that converts prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). This protein functions as a co-chaperone with heat shock protein 90 (HSP90), localizing to response elements in DNA and disrupting transcriptional activation complexes. Alternative splicing results in multiple transcript variants. There are multiple pseudogenes of this gene on several different chromosomes. [provided by RefSeq, Feb 2016].
Transcript (Including UTRs)
   Position: hg19 chr12:57,057,125-57,082,078 Size: 24,954 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr12:57,058,263-57,081,783 Size: 23,521 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr12:57,057,125-57,082,078)mRNA (may differ from genome)Protein (160 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeStanford SOURCETreefamUniProtKB
Wikipedia

-  Comments and Description Text from UniProtKB
  ID: TEBP_HUMAN
DESCRIPTION: RecName: Full=Prostaglandin E synthase 3; EC=5.3.99.3; AltName: Full=Cytosolic prostaglandin E2 synthase; Short=cPGES; AltName: Full=Hsp90 co-chaperone; AltName: Full=Progesterone receptor complex p23; AltName: Full=Telomerase-binding protein p23;
FUNCTION: Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor- mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.
CATALYTIC ACTIVITY: (5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15- hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11-alpha,15- dihydroxy-9-oxoprosta-5,13-dienoate.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=14 uM for PGH2; Vmax=190 nmol/min/mg enzyme toward PGH2;
PATHWAY: Lipid metabolism; prostaglandin biosynthesis.
SUBUNIT: Binds to the progesterone receptor. Interacts with TERT; the interaction, together with HSP90AA1, is required for correct assembly and stabilization of the telomerase holoenzyme complex.
INTERACTION: P07900:HSP90AA1; NbExp=5; IntAct=EBI-1049387, EBI-296047;
SUBCELLULAR LOCATION: Cytoplasm.
SIMILARITY: Belongs to the p23/wos2 family.
SIMILARITY: Contains 1 CS domain.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): PTGES3
CDC HuGE Published Literature: PTGES3
Positive Disease Associations: Macular Degeneration
Related Studies:
  1. Macular Degeneration
    Wei Chen et al. Proceedings of the National Academy of Sciences of the United States of America 2010, Genetic variants near TIMP3 and high-density lipoprotein-associated loci influence susceptibility to age-related macular degeneration., Proceedings of the National Academy of Sciences of the United States of America. [PubMed 20385819]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 127.01 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 3136.55 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -98.43295-0.334 Picture PostScript Text
3' UTR -293.001138-0.257 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007052 - CS-like_domain
IPR017447 - CS_domain
IPR008978 - HSP20-like_chaperone

Pfam Domains:
PF04969 - CS domain

SCOP Domains:
49764 - HSP20-like chaperones

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1EJF
- X-ray MuPIT

1LG0
- Model


ModBase Predicted Comparative 3D Structure on Q15185
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsemblFlyBase  
 Protein SequenceProtein SequenceProtein Sequence  
 AlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003720 telomerase activity
GO:0005515 protein binding
GO:0016853 isomerase activity
GO:0050220 prostaglandin-E synthase activity
GO:0051082 unfolded protein binding
GO:0051879 Hsp90 protein binding
GO:0070182 DNA polymerase binding

Biological Process:
GO:0000723 telomere maintenance
GO:0001516 prostaglandin biosynthetic process
GO:0006629 lipid metabolic process
GO:0006631 fatty acid metabolic process
GO:0006633 fatty acid biosynthetic process
GO:0006693 prostaglandin metabolic process
GO:0006805 xenobiotic metabolic process
GO:0007004 telomere maintenance via telomerase
GO:0007165 signal transduction
GO:0019371 cyclooxygenase pathway
GO:0042327 positive regulation of phosphorylation
GO:0050821 protein stabilization
GO:0051085 chaperone mediated protein folding requiring cofactor
GO:0051131 chaperone-mediated protein complex assembly
GO:0051973 positive regulation of telomerase activity
GO:1900034 regulation of cellular response to heat
GO:1905323 telomerase holoenzyme complex assembly

Cellular Component:
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005697 telomerase holoenzyme complex
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AK098214 - Homo sapiens cDNA FLJ40895 fis, clone UTERU2002294, highly similar to Prostaglandin E synthase 3 (EC 5.3.99.3).
BC003005 - Homo sapiens prostaglandin E synthase 3 (cytosolic), mRNA (cDNA clone MGC:4004 IMAGE:2821965), complete cds.
AK291945 - Homo sapiens cDNA FLJ77524 complete cds, highly similar to Homo sapiens prostaglandin E synthase 3 (cytosolic) (PTGES3), mRNA.
BC021167 - Homo sapiens prostaglandin E synthase 3 (cytosolic), mRNA (cDNA clone IMAGE:3683159), partial cds.
BC010882 - Homo sapiens cDNA clone IMAGE:3353485, **** WARNING: chimeric clone ****.
JD021073 - Sequence 2097 from Patent EP1572962.
JD287954 - Sequence 268978 from Patent EP1572962.
AK298160 - Homo sapiens cDNA FLJ50819 complete cds, highly similar to Prostaglandin E synthase 3 (EC 5.3.99.3).
JD026684 - Sequence 7708 from Patent EP1572962.
JD032444 - Sequence 13468 from Patent EP1572962.
JD513209 - Sequence 494233 from Patent EP1572962.
JD165050 - Sequence 146074 from Patent EP1572962.
JD100017 - Sequence 81041 from Patent EP1572962.
AK295208 - Homo sapiens cDNA FLJ59888 complete cds, moderately similar to Prostaglandin E synthase 3 (EC 5.3.99.3).
JD072795 - Sequence 53819 from Patent EP1572962.
AK298147 - Homo sapiens cDNA FLJ59942 complete cds, highly similar to Prostaglandin E synthase 3 (EC 5.3.99.3).
JD323395 - Sequence 304419 from Patent EP1572962.
AK293139 - Homo sapiens cDNA FLJ59832 complete cds, moderately similar to Prostaglandin E synthase 3 (EC 5.3.99.3).
L24804 - Human (p23) mRNA, complete cds.
JD246540 - Sequence 227564 from Patent EP1572962.
EU176396 - Synthetic construct Homo sapiens clone IMAGE:100006533; FLH196797.01X; RZPDo839F04252D prostaglandin E synthase 3 (cytosolic) (PTGES3) gene, encodes complete protein.
KJ893121 - Synthetic construct Homo sapiens clone ccsbBroadEn_02515 PTGES3 gene, encodes complete protein.
DQ896710 - Synthetic construct Homo sapiens clone IMAGE:100011170; FLH199368.01L; RZPDo839B0681D prostaglandin E synthase 3 (cytosolic) (PTGES3) gene, encodes complete protein.
AB464077 - Synthetic construct DNA, clone: pF1KB6813, Homo sapiens PTGES3 gene for prostaglandin E synthase 3, without stop codon, in Flexi system.
Z69891 - H.sapiens mRNA (clone ICRFp507G10101).

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY3DJ-35583 - biosynthesis of prostaglandins

Reactome (by CSHL, EBI, and GO)

Protein Q15185 (Reactome details) participates in the following event(s):

R-HSA-5618098 p23 (PTGES3) binds HSP90:ATP:FKBP5:nascent protein
R-HSA-5618110 p23 (PTGES3) binds HSP90:ATP:FKBP4:nascent protein
R-HSA-3371586 Dissociation of cytosolic HSF1:HSP90 complex
R-HSA-5324632 Dissociation of cytosolic HSF1:HSP90:HDAC6:PTGES3 upon sensing protein aggregates
R-HSA-5082409 Dissociation of HSF1:HSP90 complex in the nucleus
R-HSA-8939203 HSP90-dependent ATP hydrolysis promotes release of ESR:ESTG from chaperone complex
R-HSA-5618073 FKBP4 replaces FKBP5 within HSP90:ATP:FKBP5:unfolded protein
R-HSA-8936849 AHR:2xHSP90:AIP:PTGES3 binds TCDD
R-HSA-5618099 SH binds SHR within the HSP90 chaperone complex
R-HSA-5324617 HSP90:FKBP4:PTGES3 binds HSF1 trimer
R-HSA-8937191 AHR:TCDD:2xHSP90AB1:AIP:PTGES3 dissociates
R-HSA-8939204 ESTG binds the ESR:chaperone complex
R-HSA-265295 Prostaglandin E synthase isomerizes PGH2 to PGE2
R-HSA-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-HSA-3371511 HSF1 activation
R-HSA-8939211 ESR-mediated signaling
R-HSA-9018519 Estrogen-dependent gene expression
R-HSA-8937144 Aryl hydrocarbon receptor signalling
R-HSA-2262752 Cellular responses to stress
R-HSA-3371556 Cellular response to heat stress
R-HSA-3371568 Attenuation phase
R-HSA-9006931 Signaling by Nuclear Receptors
R-HSA-211945 Phase I - Functionalization of compounds
R-HSA-2162123 Synthesis of Prostaglandins (PG) and Thromboxanes (TX)
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-3371571 HSF1-dependent transactivation
R-HSA-162582 Signal Transduction
R-HSA-211859 Biological oxidations
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-1430728 Metabolism
R-HSA-8978868 Fatty acid metabolism
R-HSA-556833 Metabolism of lipids

-  Other Names for This Gene
  Alternate Gene Symbols: A8K7D0, NM_006601, NP_006592, P23, Q15185, Q8WU70, TEBP, TEBP_HUMAN
UCSC ID: uc001slu.4
RefSeq Accession: NM_006601
Protein: Q15185 (aka TEBP_HUMAN)
CCDS: CCDS31836.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_006601.5
exon count: 8CDS single in 3' UTR: no RNA size: 1932
ORF size: 483CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1166.00frame shift in genome: no % Coverage: 99.17
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.