Human Gene DRAP1 (uc001ogj.2) Description and Page Index
  Description: Homo sapiens DR1-associated protein 1 (negative cofactor 2 alpha) (DRAP1), mRNA.
RefSeq Summary (NM_006442): Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. [provided by RefSeq, Jul 2008]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.428529.1, SRR1163658.289450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312515.7/ ENSP00000307850.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##
Transcript (Including UTRs)
   Position: hg19 chr11:65,686,728-65,689,048 Size: 2,321 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr11:65,686,973-65,688,906 Size: 1,934 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsCTDGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr11:65,686,728-65,689,048)mRNA (may differ from genome)Protein (205 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
neXtProtOMIMPubMedReactomeStanford SOURCETreefam

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Dr1-associated corepressor; AltName: Full=Dr1-associated protein 1; AltName: Full=Negative co-factor 2-alpha; Short=NC2-alpha;
FUNCTION: The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own.
SUBUNIT: Heterodimer with DR1. Binds BTAF1.
SUBCELLULAR LOCATION: Nucleus (Potential).
TISSUE SPECIFICITY: Ubiquitous. Highly expressed in adult testis, heart, skeletal muscle, pancreas and brain, and in fetal brain, liver and kidney.
PTM: Phosphorylation reduces DNA binding, but has no effect on heterodimerization and TBP binding.
SIMILARITY: Belongs to the NC2 alpha/DRAP1 family.
SIMILARITY: Contains 1 histone-fold domain.

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 160.27 RPKM in Testis
Total median expression: 3184.19 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -130.30245-0.532 Picture PostScript Text
3' UTR -44.20142-0.311 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003958 - CBFA_NFYB_domain
IPR009072 - Histone-fold

Pfam Domains:
PF00125 - Core histone H2A/H2B/H3/H4
PF00808 - Histone-like transcription factor (CBF/NF-Y) and archaeal histone

SCOP Domains:
47113 - Histone-fold

Protein Data Bank (PDB) 3-D Structure
MuPIT help

- X-ray

ModBase Predicted Comparative 3D Structure on Q14919
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 Protein SequenceProtein SequenceProtein SequenceProtein SequenceProtein Sequence

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003714 transcription corepressor activity
GO:0005515 protein binding
GO:0042802 identical protein binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated

Cellular Component:
GO:0005634 nucleus

-  Descriptions from all associated GenBank mRNAs
  JD339143 - Sequence 320167 from Patent EP1572962.
JD068650 - Sequence 49674 from Patent EP1572962.
JD453014 - Sequence 434038 from Patent EP1572962.
BC010025 - Homo sapiens DR1-associated protein 1 (negative cofactor 2 alpha), mRNA (cDNA clone MGC:19653 IMAGE:3029273), complete cds.
JD407321 - Sequence 388345 from Patent EP1572962.
AB463581 - Synthetic construct DNA, clone: pF1KB7571, Homo sapiens DRAP1 gene for DR1-associated protein 1, without stop codon, in Flexi system.
EU446936 - Synthetic construct Homo sapiens clone IMAGE:100069794; IMAGE:100012145; FLH258550.01L DR1-associated protein 1 (negative cofactor 2 alpha) (DRAP1) gene, encodes complete protein.
CU679579 - Synthetic construct Homo sapiens gateway clone IMAGE:100018564 5' read DRAP1 mRNA.
X96506 - H.sapiens mRNA for NC2 alpha subunit.
U41843 - Human Dr1-associated corepressor (DRAP1) mRNA, complete cds.
JD216138 - Sequence 197162 from Patent EP1572962.
JD471316 - Sequence 452340 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: NC2A_HUMAN, NM_006442, NP_006433, Q13448, Q14919
UCSC ID: uc001ogj.2
RefSeq Accession: NM_006442
Protein: Q14919 (aka NC2A_HUMAN)
CCDS: CCDS8123.1

-  Gene Model Information
category: coding nonsense-mediated-decay: no RNA accession: NM_006442.3
exon count: 7CDS single in 3' UTR: no RNA size: 1022
ORF size: 618CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 1436.00frame shift in genome: no % Coverage: 98.34
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.