Human Gene ALPG (uc002vss.4)
  Description: Homo sapiens alkaline phosphatase, placental-like 2 (ALPG), mRNA.
RefSeq Summary (NM_031313): There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme, localized to testis, thymus and certain germ cell tumors, that is closely related to both the placental and intestinal forms of alkaline phosphatase. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr2:233,271,552-233,275,424 Size: 3,873 Total Exon Count: 11 Strand: +
Coding Region
   Position: hg19 chr2:233,271,605-233,274,582 Size: 2,978 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:233,271,552-233,275,424)mRNA (may differ from genome)Protein (532 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsGeneNetwork
H-INVHPRDLynxMGIneXtProtOMIM
PubMedReactomeTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PPBN_HUMAN
DESCRIPTION: RecName: Full=Alkaline phosphatase, placental-like; EC=3.1.3.1; AltName: Full=ALP-1; AltName: Full=Alkaline phosphatase Nagao isozyme; AltName: Full=Germ cell alkaline phosphatase; Short=GCAP; AltName: Full=Placental alkaline phosphatase-like; Short=PLAP-like; Flags: Precursor;
CATALYTIC ACTIVITY: A phosphate monoester + H(2)O = an alcohol + phosphate.
COFACTOR: Binds 1 magnesium ion (By similarity).
COFACTOR: Binds 2 zinc ions (By similarity).
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
TISSUE SPECIFICITY: Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors.
MISCELLANEOUS: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
SIMILARITY: Belongs to the alkaline phosphatase family.
SEQUENCE CAUTION: Sequence=CAA30232.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.31 RPKM in Testis
Total median expression: 0.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -7.5053-0.142 Picture PostScript Text
3' UTR -328.64842-0.390 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017849 - Alkaline_Pase-like_a/b/a
IPR001952 - Alkaline_phosphatase
IPR018299 - Alkaline_phosphatase_AS
IPR017850 - Alkaline_phosphatase_core

Pfam Domains:
PF00245 - Alkaline phosphatase

SCOP Domains:
53649 - Alkaline phosphatase-like

ModBase Predicted Comparative 3D Structure on P10696
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologNo orthologGenome Browser
 Gene Details   Gene Details
 Gene Sorter   Gene Sorter
 RGD   SGD
 Protein Sequence   Protein Sequence
 Alignment   Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004035 alkaline phosphatase activity
GO:0016787 hydrolase activity
GO:0016791 phosphatase activity
GO:0046872 metal ion binding

Biological Process:
GO:0008152 metabolic process
GO:0016311 dephosphorylation

Cellular Component:
GO:0005576 extracellular region
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0031225 anchored component of membrane


-  Descriptions from all associated GenBank mRNAs
  AK297894 - Homo sapiens cDNA FLJ61142 complete cds, highly similar to Alkaline phosphatase, placental type precursor(EC 3.1.3.1).
J04948 - Human alkaline phosphatase (ALP-1) mRNA, complete cds.
AK293017 - Homo sapiens cDNA FLJ75984 complete cds, highly similar to Homo sapiens alkaline phosphatase, placental-like 2 (ALPPL2), mRNA.
BC014139 - Homo sapiens alkaline phosphatase, placental-like 2, mRNA (cDNA clone MGC:20678 IMAGE:4652865), complete cds.
X55958 - H.sapiens mRNA for alkaline phosphatase.
X53279 - Human mRNA for placental-like alkaline phosphatase (EC 3.1.3.1).
KJ896415 - Synthetic construct Homo sapiens clone ccsbBroadEn_05809 ALPPL2 gene, encodes complete protein.
AB012642 - Homo sapiens mRNA for alkaline phosphatase, partial cds.
JD365668 - Sequence 346692 from Patent EP1572962.
JD127800 - Sequence 108824 from Patent EP1572962.
JD275147 - Sequence 256171 from Patent EP1572962.
JD342887 - Sequence 323911 from Patent EP1572962.
JD156819 - Sequence 137843 from Patent EP1572962.
JD269448 - Sequence 250472 from Patent EP1572962.
JD189439 - Sequence 170463 from Patent EP1572962.
JD392590 - Sequence 373614 from Patent EP1572962.
JD445194 - Sequence 426218 from Patent EP1572962.
JD384333 - Sequence 365357 from Patent EP1572962.
JD054942 - Sequence 35966 from Patent EP1572962.
JD365365 - Sequence 346389 from Patent EP1572962.
JD178196 - Sequence 159220 from Patent EP1572962.
JD205439 - Sequence 186463 from Patent EP1572962.
JD349053 - Sequence 330077 from Patent EP1572962.
JD378739 - Sequence 359763 from Patent EP1572962.
JD406242 - Sequence 387266 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00790 - Folate biosynthesis
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein P10696 (Reactome details) participates in the following event(s):

R-HSA-8940388 GPLD1 hydrolyses GPI-anchors from proteins
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A8KAF2, ALPPL, ALPPL2, NM_031313, NP_112603, P10696, PPBN_HUMAN, Q16727, Q53S81, Q96CM1
UCSC ID: uc002vss.4
RefSeq Accession: NM_031313
Protein: P10696 (aka PPBN_HUMAN)
CCDS: CCDS2491.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_031313.2
exon count: 11CDS single in 3' UTR: no RNA size: 2499
ORF size: 1599CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3258.00frame shift in genome: no % Coverage: 99.80
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.