Human Gene DCAF10 (uc004aao.3)
  Description: Homo sapiens DDB1 and CUL4 associated factor 10 (DCAF10), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr9:37,800,790-37,867,665 Size: 66,876 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr9:37,800,864-37,861,505 Size: 60,642 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:37,800,790-37,867,665)mRNA (may differ from genome)Protein (559 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkH-INVHGNCHPRDLynxMGI
neXtProtPubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: DCA10_HUMAN
DESCRIPTION: RecName: Full=DDB1- and CUL4-associated factor 10; AltName: Full=WD repeat-containing protein 32;
FUNCTION: May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Interacts with DDB1.
SIMILARITY: Belongs to the WD repeat DCAF10 family.
SIMILARITY: Contains 7 WD repeats.
SEQUENCE CAUTION: Sequence=AAI08687.1; Type=Erroneous initiation; Sequence=BAB15574.1; Type=Erroneous initiation; Sequence=BAC11043.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 12.39 RPKM in Testis
Total median expression: 231.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -27.4074-0.370 Picture PostScript Text
3' UTR -1724.066160-0.280 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR019775 - WD40_repeat_CS
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Prolyl oligopeptidase, N-terminal domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - Dipeptidyl peptidase IV/CD26, N-terminal domain

ModBase Predicted Comparative 3D Structure on Q5QP82
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserGenome BrowserNo orthologNo ortholog
Gene Details  Gene Details  
Gene Sorter  Gene Sorter  
  EnsemblFlyBase  
  Protein SequenceProtein Sequence  
  AlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Biological Process:
GO:0016567 protein ubiquitination
GO:0043687 post-translational protein modification

Cellular Component:
GO:0005654 nucleoplasm
GO:0080008 Cul4-RING E3 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  AK309712 - Homo sapiens cDNA, FLJ99753.
BC108686 - Homo sapiens WD repeat domain 32, mRNA (cDNA clone MGC:131781 IMAGE:6157119), complete cds.
BC139834 - Homo sapiens WD repeat domain 32, mRNA (cDNA clone IMAGE:40116382), partial cds.
AK074532 - Homo sapiens cDNA FLJ90051 fis, clone HEMBA1002551, weakly similar to PUTATIVE SERINE/THREONINE-PROTEIN KINASE PKWA (EC 2.7.1.-).
AK315756 - Homo sapiens cDNA, FLJ96868.
BC011959 - Homo sapiens WD repeat domain 32, mRNA (cDNA clone IMAGE:3861517), partial cds.
AK026854 - Homo sapiens cDNA: FLJ23201 fis, clone KAIA38872.
BC003520 - Homo sapiens WD repeat domain 32, mRNA (cDNA clone IMAGE:3606341), partial cds.
CU678476 - Synthetic construct Homo sapiens gateway clone IMAGE:100018045 5' read WDR32 mRNA.
CU680130 - Synthetic construct Homo sapiens gateway clone IMAGE:100020637 5' read WDR32 mRNA.
KJ903165 - Synthetic construct Homo sapiens clone ccsbBroadEn_12559 DCAF10 gene, encodes complete protein.
KJ903166 - Synthetic construct Homo sapiens clone ccsbBroadEn_12560 DCAF10 gene, encodes complete protein.
JD353784 - Sequence 334808 from Patent EP1572962.
JD555645 - Sequence 536669 from Patent EP1572962.
JD423891 - Sequence 404915 from Patent EP1572962.
JD162341 - Sequence 143365 from Patent EP1572962.
JD508712 - Sequence 489736 from Patent EP1572962.
JD198777 - Sequence 179801 from Patent EP1572962.
JD245235 - Sequence 226259 from Patent EP1572962.
JD259949 - Sequence 240973 from Patent EP1572962.
JD438768 - Sequence 419792 from Patent EP1572962.
JD445600 - Sequence 426624 from Patent EP1572962.
JD038781 - Sequence 19805 from Patent EP1572962.
JD244479 - Sequence 225503 from Patent EP1572962.
JD468545 - Sequence 449569 from Patent EP1572962.
JD320478 - Sequence 301502 from Patent EP1572962.
JD113067 - Sequence 94091 from Patent EP1572962.
JD510861 - Sequence 491885 from Patent EP1572962.
JD260558 - Sequence 241582 from Patent EP1572962.
JD105333 - Sequence 86357 from Patent EP1572962.
JD420198 - Sequence 401222 from Patent EP1572962.
JD264173 - Sequence 245197 from Patent EP1572962.
JD438879 - Sequence 419903 from Patent EP1572962.
JD208780 - Sequence 189804 from Patent EP1572962.
JD275740 - Sequence 256764 from Patent EP1572962.
JD112151 - Sequence 93175 from Patent EP1572962.
JD395790 - Sequence 376814 from Patent EP1572962.
JD217819 - Sequence 198843 from Patent EP1572962.
JD144734 - Sequence 125758 from Patent EP1572962.
JD197989 - Sequence 179013 from Patent EP1572962.
JD135721 - Sequence 116745 from Patent EP1572962.
JD135722 - Sequence 116746 from Patent EP1572962.
JD238311 - Sequence 219335 from Patent EP1572962.
JD238310 - Sequence 219334 from Patent EP1572962.
JD457006 - Sequence 438030 from Patent EP1572962.
JD117499 - Sequence 98523 from Patent EP1572962.
JD554160 - Sequence 535184 from Patent EP1572962.
JD084395 - Sequence 65419 from Patent EP1572962.
JD430298 - Sequence 411322 from Patent EP1572962.
JD529385 - Sequence 510409 from Patent EP1572962.
JD169632 - Sequence 150656 from Patent EP1572962.
JD306301 - Sequence 287325 from Patent EP1572962.
JD055173 - Sequence 36197 from Patent EP1572962.
JD125595 - Sequence 106619 from Patent EP1572962.
JD125596 - Sequence 106620 from Patent EP1572962.
JD395408 - Sequence 376432 from Patent EP1572962.
JD019208 - Sequence 232 from Patent EP1572962.
JD030208 - Sequence 11232 from Patent EP1572962.
JD286547 - Sequence 267571 from Patent EP1572962.
JD511408 - Sequence 492432 from Patent EP1572962.
JD317526 - Sequence 298550 from Patent EP1572962.
AK023620 - Homo sapiens cDNA FLJ13558 fis, clone PLACE1007743.
JD020524 - Sequence 1548 from Patent EP1572962.
JD028289 - Sequence 9313 from Patent EP1572962.
JD026782 - Sequence 7806 from Patent EP1572962.
JD033182 - Sequence 14206 from Patent EP1572962.
JD020240 - Sequence 1264 from Patent EP1572962.
JD035910 - Sequence 16934 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q5QP82 (Reactome details) participates in the following event(s):

R-HSA-8955245 CAND1 binds CRL4 E3 ubiquitin ligase in the nucleus
R-HSA-8955285 COMMDs displace CAND1 from CRL4 E3 ubiquitin ligase complex
R-HSA-8952639 NEDD8:AcM-UBE2M binds CRL4 E3 ubiquitin ligase complex
R-HSA-8956045 COP9 signalosome deneddylates nuclear CRL4 E3 ubiquitin ligase complex
R-HSA-8952638 AcM-UBE2M transfers NEDD8 to CRL4 E3 ubiquitin ligase complex
R-HSA-8951664 Neddylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A4VCJ5, DCA10_HUMAN, NM_024345, NP_077321, Q32NE2, Q5QP82, Q8N2Q5, Q96ET5, Q9BTQ5, Q9H5P6, WDR32
UCSC ID: uc004aao.3
RefSeq Accession: NM_024345
Protein: Q5QP82 (aka DCA10_HUMAN)
CCDS: CCDS6613.2

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_024345.3
exon count: 7CDS single in 3' UTR: no RNA size: 7932
ORF size: 1680CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3560.00frame shift in genome: no % Coverage: 99.77
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.