Human Gene SLC9B1 (uc003hww.3)
  Description: Homo sapiens solute carrier family 9, subfamily B (NHA1, cation proton antiporter 1), member 1 (SLC9B1), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.
RefSeq Summary (NM_139173): The protein encoded by this gene is a sodium/hydrogen exchanger and transmembrane protein. Highly conserved orthologs of this gene have been found in other mammalian species. The expression of this gene may be limited to testis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010].
Transcript (Including UTRs)
   Position: hg19 chr4:103,822,085-103,940,896 Size: 118,812 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr4:103,822,274-103,912,868 Size: 90,595 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:103,822,085-103,940,896)mRNA (may differ from genome)Protein (515 aa)
Gene SorterGenome BrowserOther Species FASTATable SchemaAlphaFoldBioGPS
EnsemblEntrez GeneExonPrimerGeneCardsGeneNetworkH-INV
HGNCHPRDLynxMGIneXtProtOMIM
PubMedReactomeTreefamUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: SL9B1_HUMAN
DESCRIPTION: RecName: Full=Sodium/hydrogen exchanger 9B1; AltName: Full=Na(+)/H(+) exchanger-like domain-containing protein 1; Short=NHE domain-containing protein 1; AltName: Full=Sodium/hydrogen exchanger-like domain-containing protein 1; AltName: Full=Solute carrier family 9 subfamily B member 1;
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential).
TISSUE SPECIFICITY: Expressed only in the testis.
SIMILARITY: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
SEQUENCE CAUTION: Sequence=AAM22868.1; Type=Frameshift; Positions=219, 298, 381;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 20.40 RPKM in Testis
Total median expression: 30.84 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -64.00142-0.451 Picture PostScript Text
3' UTR -32.91189-0.174 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR006153 - Cation/H_exchanger

Pfam Domains:
PF00999 - Sodium/hydrogen exchanger family

ModBase Predicted Comparative 3D Structure on Q4ZJI4
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene Details   Gene Details 
Gene Sorter   Gene Sorter 
    WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0015297 antiporter activity
GO:0015299 solute:proton antiporter activity
GO:0015385 sodium:proton antiporter activity

Biological Process:
GO:0006811 ion transport
GO:0006812 cation transport
GO:0006814 sodium ion transport
GO:0007338 single fertilization
GO:0030317 flagellated sperm motility
GO:0034220 ion transmembrane transport
GO:0035725 sodium ion transmembrane transport
GO:0051453 regulation of intracellular pH
GO:0055085 transmembrane transport
GO:1902600 hydrogen ion transmembrane transport

Cellular Component:
GO:0005886 plasma membrane
GO:0005929 cilium
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031514 motile cilium
GO:0042995 cell projection
GO:0097228 sperm principal piece


-  Descriptions from all associated GenBank mRNAs
  BC022079 - Homo sapiens Na+/H+ exchanger domain containing 1, mRNA (cDNA clone IMAGE:4823090).
BC046636 - Homo sapiens Na+/H+ exchanger domain containing 1, mRNA (cDNA clone IMAGE:5296769).
HM005440 - Homo sapiens clone HTL-T-127 testicular tissue protein Li 127 mRNA, complete cds.
AY461581 - Homo sapiens Na-H-exchanger protein mRNA, complete cds.
DQ003059 - Homo sapiens NHE domain-containing 1 protein (NHEDC1) mRNA, complete cds.
BC132712 - Homo sapiens Na+/H+ exchanger domain containing 1, mRNA (cDNA clone MGC:164343 IMAGE:40146734), complete cds.
BC136966 - Homo sapiens Na+/H+ exchanger domain containing 1, mRNA (cDNA clone MGC:168581 IMAGE:9020958), complete cds.
BC144443 - Homo sapiens cDNA clone IMAGE:9052968.
BC110794 - Homo sapiens Na+/H+ exchanger domain containing 1, mRNA (cDNA clone MGC:131641 IMAGE:5745227), complete cds.
AF447585 - Homo sapiens tissue-type testis unknown mRNA.
AK313592 - Homo sapiens cDNA, FLJ94158, highly similar to Homo sapiens CG10806-like (LOC150159), mRNA.
HQ258576 - Synthetic construct Homo sapiens clone IMAGE:100073102 Na+/H+ exchanger domain containing 1 (NHEDC1) gene, encodes complete protein.
KJ900272 - Synthetic construct Homo sapiens clone ccsbBroadEn_09666 SLC9B1 gene, encodes complete protein.
JD038628 - Sequence 19652 from Patent EP1572962.
KJ903865 - Synthetic construct Homo sapiens clone ccsbBroadEn_13259 SLC9B1 gene, encodes complete protein.
JD233637 - Sequence 214661 from Patent EP1572962.
JD546484 - Sequence 527508 from Patent EP1572962.
JD295958 - Sequence 276982 from Patent EP1572962.
JD550659 - Sequence 531683 from Patent EP1572962.
JD456676 - Sequence 437700 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q4ZJI4 (Reactome details) participates in the following event(s):

R-HSA-2872444 SLC9B1/C2 exchange Na+ for H+
R-HSA-2672351 Stimuli-sensing channels
R-HSA-983712 Ion channel transport
R-HSA-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: A1KXV1, B9EH04, C9JBP7, NHEDC1, NM_139173, NP_631912, Q49A30, Q4ZJI4, Q8NCV2, Q8WVZ0, SL9B1_HUMAN
UCSC ID: uc003hww.3
RefSeq Accession: NM_139173
Protein: Q4ZJI4 (aka SL9B1_HUMAN)
CCDS: CCDS34041.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_139173.3
exon count: 12CDS single in 3' UTR: no RNA size: 1879
ORF size: 1548CDS single in intron: no Alignment % ID: 99.95
txCdsPredict score: 3282.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.