Human Gene DPEP1 (uc002fns.4) Description and Page Index
  Description: Homo sapiens dipeptidase 1 (renal) (DPEP1), transcript variant 2, mRNA.
RefSeq Summary (NM_004413): The protein encoded by this gene is a kidney membrane enzyme involved in the metabolism of glutathione and other similar proteins by dipeptide hydrolysis. The encoded protein is known to regulate leukotriene activity by catalyzing the conversion of leukotriene D4 to leukotriene E4. This protein uses zinc as a cofactor and acts as a disulfide-linked homodimer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2012].
Transcript (Including UTRs)
   Position: hg19 chr16:89,696,697-89,704,839 Size: 8,143 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr16:89,696,819-89,704,630 Size: 7,812 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsGenetic AssociationsCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:89,696,697-89,704,839)mRNA (may differ from genome)Protein (411 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
BioGPSCGAPEnsemblEntrez GeneExonPrimerGeneCards
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UniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: DPEP1_HUMAN
DESCRIPTION: RecName: Full=Dipeptidase 1; EC=3.4.13.19; AltName: Full=Dehydropeptidase-I; AltName: Full=Microsomal dipeptidase; AltName: Full=Renal dipeptidase; Short=hRDP; Flags: Precursor;
FUNCTION: Hydrolyzes a wide range of dipeptides. Implicated in the renal metabolism of glutathione and its conjugates. Converts leukotriene D4 to leukotriene E4; it may play an important role in the regulation of leukotriene activity.
CATALYTIC ACTIVITY: Hydrolysis of dipeptides.
COFACTOR: Zinc.
ENZYME REGULATION: Inhibited by L-penicillamine (By similarity).
SUBUNIT: Homodimer; disulfide-linked.
INTERACTION: Q96EK5:KIAA1279; NbExp=2; IntAct=EBI-749514, EBI-744150;
SUBCELLULAR LOCATION: Apical cell membrane; Lipid-anchor, GPI- anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI- anchor. Note=Brush border membrane.
SIMILARITY: Belongs to the peptidase M19 family.

-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): DPEP1
CDC HuGE Published Literature: DPEP1

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 265.96 RPKM in Small Intestine - Terminal Ileum
Total median expression: 503.43 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -49.50122-0.406 Picture PostScript Text
3' UTR -81.20209-0.389 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000180 - Pept_M19_AS
IPR008257 - Peptidase_M19

Pfam Domains:
PF01244 - Membrane dipeptidase (Peptidase family M19)

SCOP Domains:
51556 - Metallo-dependent hydrolases

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1ITQ
- X-ray MuPIT

1ITU
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P16444
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserGenome BrowserNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsemblFlyBase  
 Protein SequenceProtein SequenceProtein Sequence  
 AlignmentAlignmentAlignment  

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008235 metalloexopeptidase activity
GO:0008237 metallopeptidase activity
GO:0008239 dipeptidyl-peptidase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016805 dipeptidase activity
GO:0034235 GPI anchor binding
GO:0043027 cysteine-type endopeptidase inhibitor activity involved in apoptotic process
GO:0046872 metal ion binding
GO:0070573 metallodipeptidase activity
GO:0072341 modified amino acid binding

Biological Process:
GO:0006508 proteolysis
GO:0006691 leukotriene metabolic process
GO:0006749 glutathione metabolic process
GO:0006805 xenobiotic metabolic process
GO:0006915 apoptotic process
GO:0016999 antibiotic metabolic process
GO:0030336 negative regulation of cell migration
GO:0035690 cellular response to drug
GO:0043066 negative regulation of apoptotic process
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0050667 homocysteine metabolic process
GO:0071277 cellular response to calcium ion
GO:0071732 cellular response to nitric oxide
GO:0072340 cellular lactam catabolic process

Cellular Component:
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016324 apical plasma membrane
GO:0030054 cell junction
GO:0031225 anchored component of membrane
GO:0031528 microvillus membrane
GO:0042995 cell projection
GO:0045177 apical part of cell
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK309577 - Homo sapiens cDNA, FLJ99618.
J05257 - Homo sapiens (clones MDP4, MDP7) microsomal dipeptidase (MDP) mRNA, complete cds.
BC017023 - Homo sapiens dipeptidase 1 (renal), mRNA (cDNA clone MGC:8990 IMAGE:3846046), complete cds.
JD100806 - Sequence 81830 from Patent EP1572962.
JD075408 - Sequence 56432 from Patent EP1572962.
D13138 - Homo sapiens mRNA for dipeptidase precursor, complete cds.
E04234 - DNA encoding human dehydropeptidase.
JD021406 - Sequence 2430 from Patent EP1572962.
JD081116 - Sequence 62140 from Patent EP1572962.
HM005370 - Homo sapiens clone HTL-T-57 testicular tissue protein Li 57 mRNA, complete cds.
DQ890714 - Synthetic construct clone IMAGE:100003344; FLH165389.01X; RZPDo839B09160D dipeptidase 1 (renal) (DPEP1) gene, encodes complete protein.
EU832223 - Synthetic construct Homo sapiens clone HAIB:100067252; DKFZo004D0826 dipeptidase 1 (renal) protein (DPEP1) gene, encodes complete protein.
EU832130 - Synthetic construct Homo sapiens clone HAIB:100067159; DKFZo008D0825 dipeptidase 1 (renal) protein (DPEP1) gene, encodes complete protein.
AB590480 - Synthetic construct DNA, clone: pFN21AE1306, Homo sapiens DPEP1 gene for dipeptidase 1, without stop codon, in Flexi system.
KJ891064 - Synthetic construct Homo sapiens clone ccsbBroadEn_00458 DPEP1 gene, encodes complete protein.
CR457404 - Homo sapiens full open reading frame cDNA clone RZPDo834C0519D for gene DPEP1, dipeptidase 1 (renal); complete cds, incl. stopcodon.
BT006664 - Homo sapiens dipeptidase 1 (renal) mRNA, complete cds.
JD036184 - Sequence 17208 from Patent EP1572962.
JD403655 - Sequence 384679 from Patent EP1572962.
JD128705 - Sequence 109729 from Patent EP1572962.
JD388007 - Sequence 369031 from Patent EP1572962.
JD332839 - Sequence 313863 from Patent EP1572962.
JD205803 - Sequence 186827 from Patent EP1572962.
JD097298 - Sequence 78322 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P16444 (Reactome details) participates in the following event(s):

R-HSA-266012 LTD4 is converted to LTE4 by DPEP1/2
R-HSA-5433067 DPEPs hydrolyse glycine from AFXBO-CG, AFNBO-CG
R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)
R-HSA-5423646 Aflatoxin activation and detoxification
R-HSA-2142753 Arachidonic acid metabolism
R-HSA-211859 Biological oxidations
R-HSA-8978868 Fatty acid metabolism
R-HSA-1430728 Metabolism
R-HSA-556833 Metabolism of lipids

-  Other Names for This Gene
  Alternate Gene Symbols: BC017023, D3DX80, DPEP1_HUMAN, MDP, NM_004413, NP_004404, P16444, Q96AK2, RDP
UCSC ID: uc002fns.4
RefSeq Accession: NM_004413
Protein: P16444 (aka DPEP1_HUMAN)
CCDS: CCDS10982.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: BC017023.1
exon count: 10CDS single in 3' UTR: no RNA size: 1583
ORF size: 1236CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2672.00frame shift in genome: no % Coverage: 98.99
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 16# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.