Human Gene BRF1 (ENST00000619151.4) Description and Page Index
  Description: BRF1, RNA polymerase III transcription initiation factor subunit (from HGNC BRF1)
RefSeq Summary (NM_001519): This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011].
Gencode Transcript: ENST00000619151.4
Gencode Gene: ENSG00000185024.17
Transcript (Including UTRs)
   Position: hg38 chr14:105,209,496-105,300,992 Size: 91,497 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg38 chr14:105,209,496-105,241,346 Size: 31,851 Coding Exon Count: 6 

Page IndexSequence and LinksMalaCardsCTDRNA-Seq ExpressionMicroarray Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathways
Other NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:105,209,496-105,300,992)mRNA (may differ from genome)Protein (184 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblExonPrimerGeneCardsHGNCLynx
MGIPubMedStanford SOURCEUniProtKBWikipedia

-  MalaCards Disease Associations
  MalaCards Gene Search: BRF1
Diseases sorted by gene-association score: cerebellofaciodental syndrome* (1579), adult syndrome (9), hemifacial microsomia (5), chronic rhinitis (5)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.05 RPKM in Brain - Cerebellum
Total median expression: 337.67 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -498.00975-0.511 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013763 - Cyclin-like
IPR000812 - TFIIB
IPR013150 - TFIIB_cyclin

Pfam Domains:
PF00382 - Transcription factor TFIIB repeat

ModBase Predicted Comparative 3D Structure on A3KN61
FrontTopSide
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001026 TFIIIB-type transcription factor activity
GO:0017025 TBP-class protein binding

Biological Process:
GO:0006355 regulation of transcription, DNA-templated
GO:0006359 regulation of transcription from RNA polymerase III promoter
GO:0006383 transcription from RNA polymerase III promoter
GO:0070897 DNA-templated transcriptional preinitiation complex assembly

Cellular Component:
GO:0000126 transcription factor TFIIIB complex


-  Descriptions from all associated GenBank mRNAs
  LF385530 - JP 2014500723-A/193033: Polycomb-Associated Non-Coding RNAs.
MA621107 - JP 2018138019-A/193033: Polycomb-Associated Non-Coding RNAs.
U28838 - Human transcription factor TFIIIB 90 kDa subunit (hTFIIIB90) mRNA, complete cds.
BC086856 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone MGC:105048 IMAGE:6166751), complete cds.
U75276 - Human TFIIB related factor hBRF (HBRF) mRNA, complete cds.
AK295579 - Homo sapiens cDNA FLJ58798 complete cds, highly similar to Transcription factor IIIB 90 kDa subunit.
AK294899 - Homo sapiens cDNA FLJ58760 complete cds, highly similar to Transcription factor IIIB 90 kDa subunit.
AB527272 - Synthetic construct DNA, clone: pF1KB3029, Homo sapiens BRF1 gene for BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB, without stop codon, in Flexi system.
BC071637 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:4177747), partial cds.
AK126499 - Homo sapiens cDNA FLJ44535 fis, clone UTERU3004709.
AK125024 - Homo sapiens cDNA FLJ43034 fis, clone BRTHA3001721, highly similar to Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1).
AK124665 - Homo sapiens cDNA FLJ42674 fis, clone BRAMY2029988, highly similar to Transcription factor IIIB 90 kDa subunit.
AJ297407 - Homo sapiens mRNA for transcription factor II B-related factor, isoform 3 (BRF gene).
AJ297406 - Homo sapiens mRNA for transcription factor II B-related factor, isoform 2 (BRF gene).
AK096471 - Homo sapiens cDNA FLJ39152 fis, clone OCBBF2001910, highly similar to Transcription factor IIIB 90 kDa subunit.
BC103859 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:40003193), complete cds.
BC103860 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:40003197), complete cds.
BC103861 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:40003203), complete cds.
BC133655 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:40003194), complete cds.
JD429505 - Sequence 410529 from Patent EP1572962.
JD137312 - Sequence 118336 from Patent EP1572962.
JD376424 - Sequence 357448 from Patent EP1572962.
JD344369 - Sequence 325393 from Patent EP1572962.
JD055375 - Sequence 36399 from Patent EP1572962.
JD439760 - Sequence 420784 from Patent EP1572962.
JD329421 - Sequence 310445 from Patent EP1572962.
BC016743 - Homo sapiens BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), mRNA (cDNA clone IMAGE:4830425), complete cds.
EU668327 - Homo sapiens epididymis secretory sperm binding protein Li 76p (HEL-S-76p) mRNA, complete cds.
AJ297408 - Homo sapiens mRNA for partial transcription factor II B-related factor, isoform 4 (BRF gene).
CU677703 - Synthetic construct Homo sapiens gateway clone IMAGE:100018580 5' read BRF1 mRNA.
JD462556 - Sequence 443580 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_RNApol3Pathway - RNA polymerase III transcription

-  Other Names for This Gene
  Alternate Gene Symbols: A3KN61, A3KN61_HUMAN, U28838, uc059fzf.1
UCSC ID: uc059fzf.1
RefSeq Accession: NM_001519
Protein: A3KN61

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.