Human Gene BID (ENST00000551952.5) Description and Page Index
  Description: Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 4, mRNA. (from RefSeq NM_001244567)
RefSeq Summary (NM_001244567): This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jan 2020].
Gencode Transcript: ENST00000551952.5
Gencode Gene: ENSG00000015475.18
Transcript (Including UTRs)
   Position: hg38 chr22:17,735,552-17,774,665 Size: 39,114 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg38 chr22:17,735,580-17,750,116 Size: 14,537 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:17,735,552-17,774,665)mRNA (may differ from genome)Protein (195 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
LynxMGIneXtProtOMIMPubMedReactome
Stanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: BID_HUMAN
DESCRIPTION: RecName: Full=BH3-interacting domain death agonist; AltName: Full=p22 BID; Short=BID; Contains: RecName: Full=BH3-interacting domain death agonist p15; AltName: Full=p15 BID; Contains: RecName: Full=BH3-interacting domain death agonist p13; AltName: Full=p13 BID; Contains: RecName: Full=BH3-interacting domain death agonist p11; AltName: Full=p11 BID;
FUNCTION: The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
SUBUNIT: Forms heterodimers either with the pro-apoptotic protein BAX or the anti-apoptotic protein Bcl-2 (By similarity). p15 BID interacts with ITCH.
INTERACTION: Q07812:BAX; NbExp=14; IntAct=EBI-519672, EBI-516580; P10415:BCL2; NbExp=8; IntAct=EBI-519672, EBI-77694; Q07440:Bcl2a1 (xeno); NbExp=3; IntAct=EBI-519672, EBI-707754; Q07817-1:BCL2L1; NbExp=6; IntAct=EBI-519672, EBI-287195; Q92843:BCL2L2; NbExp=5; IntAct=EBI-519672, EBI-707714; Q03135:CAV1; NbExp=3; IntAct=EBI-519672, EBI-603614;
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Mitochondrion membrane (By similarity). Note=When uncleaved, it is predominantly cytoplasmic.
SUBCELLULAR LOCATION: BH3-interacting domain death agonist p15: Mitochondrion membrane (By similarity). Note=Translocates to mitochondria as an integral membrane protein (By similarity).
SUBCELLULAR LOCATION: BH3-interacting domain death agonist p13: Mitochondrion membrane (By similarity). Note=Associated with the mitochondrial membrane (By similarity).
SUBCELLULAR LOCATION: Isoform 1: Cytoplasm.
SUBCELLULAR LOCATION: Isoform 3: Cytoplasm.
SUBCELLULAR LOCATION: Isoform 2: Mitochondrion membrane. Note=A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively.
TISSUE SPECIFICITY: Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
DOMAIN: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
PTM: TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
PTM: p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
SEQUENCE CAUTION: Sequence=AAH22072.2; Type=Erroneous initiation;
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/bid/";

-  MalaCards Disease Associations
  MalaCards Gene Search: BID
Diseases sorted by gene-association score: alzheimer disease mitochondrial (2), cat eye syndrome (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 34.09 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 454.81 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -28.9084-0.344 Picture PostScript Text
3' UTR -5.7028-0.204 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020728 - Bcl2_BH3_motif_CS
IPR010479 - BID

Pfam Domains:
PF06393 - BH3 interacting domain (BID)

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1ZY3
- NMR MuPIT

2BID
- NMR MuPIT

2KBW
- NMR MuPIT


ModBase Predicted Comparative 3D Structure on P55957
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005123 death receptor binding
GO:0005515 protein binding
GO:0031625 ubiquitin protein ligase binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0001836 release of cytochrome c from mitochondria
GO:0006626 protein targeting to mitochondrion
GO:0006915 apoptotic process
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors
GO:0008637 apoptotic mitochondrial changes
GO:0010918 positive regulation of mitochondrial membrane potential
GO:0032459 regulation of protein oligomerization
GO:0032461 positive regulation of protein oligomerization
GO:0032464 positive regulation of protein homooligomerization
GO:0042127 regulation of cell proliferation
GO:0042770 signal transduction in response to DNA damage
GO:0042775 mitochondrial ATP synthesis coupled electron transport
GO:0042981 regulation of apoptotic process
GO:0043065 positive regulation of apoptotic process
GO:0043066 negative regulation of apoptotic process
GO:0051260 protein homooligomerization
GO:0051402 neuron apoptotic process
GO:0090150 establishment of protein localization to membrane
GO:0090200 positive regulation of release of cytochrome c from mitochondria
GO:0097191 extrinsic apoptotic signaling pathway
GO:0097284 hepatocyte apoptotic process
GO:0097345 mitochondrial outer membrane permeabilization
GO:1900740 positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
GO:1901030 positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
GO:1902108 regulation of mitochondrial membrane permeability involved in apoptotic process
GO:1902230 negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
GO:2000045 regulation of G1/S transition of mitotic cell cycle
GO:2000271 positive regulation of fibroblast apoptotic process
GO:2001235 positive regulation of apoptotic signaling pathway
GO:2001238 positive regulation of extrinsic apoptotic signaling pathway
GO:2001244 positive regulation of intrinsic apoptotic signaling pathway

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005741 mitochondrial outer membrane
GO:0005829 cytosol
GO:0016020 membrane
GO:0031966 mitochondrial membrane
GO:0032592 integral component of mitochondrial membrane


-  Descriptions from all associated GenBank mRNAs
  BC036364 - Homo sapiens BH3 interacting domain death agonist, mRNA (cDNA clone MGC:42355 IMAGE:4819949), complete cds.
LF384185 - JP 2014500723-A/191688: Polycomb-Associated Non-Coding RNAs.
MA619762 - JP 2018138019-A/191688: Polycomb-Associated Non-Coding RNAs.
BC009197 - Homo sapiens BH3 interacting domain death agonist, mRNA (cDNA clone MGC:15319 IMAGE:4025880), complete cds.
AK094795 - Homo sapiens cDNA FLJ37476 fis, clone BRAWH2012827, highly similar to Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 1, mRNA.
D89803 - Homo sapiens mRNA for desmocollin type 4, complete cds.
AF042083 - Homo sapiens BH3 interacting domain death agonist (BID) mRNA, complete cds.
AF370380 - Homo sapiens FP497 mRNA, complete cds.
BC033634 - Homo sapiens BH3 interacting domain death agonist, mRNA (cDNA clone MGC:45290 IMAGE:5538243), complete cds.
AF087891 - Homo sapiens apoptic death agonist BID mRNA, complete cds.
BC022072 - Homo sapiens BH3 interacting domain death agonist, mRNA (cDNA clone IMAGE:4797862), partial cds.
AF250233 - Homo sapiens apoptotic regulator Bid isoform EL (BID) mRNA, complete cds.
AY005151 - Homo sapiens BID S (BID) mRNA, complete cds.
CR456389 - Homo sapiens BID full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.BID).
EU678292 - Homo sapiens BID isoform Si6 (BID) mRNA, complete cds, alternatively spliced.
EU678293 - Homo sapiens BID isoform ES(1b) (BID) mRNA, complete cds, alternatively spliced.
EU678294 - Homo sapiens BID isoform L(2) (BID) mRNA, complete cds, alternatively spliced.
JD305510 - Sequence 286534 from Patent EP1572962.
LF365624 - JP 2014500723-A/173127: Polycomb-Associated Non-Coding RNAs.
MA601201 - JP 2018138019-A/173127: Polycomb-Associated Non-Coding RNAs.
KJ890767 - Synthetic construct Homo sapiens clone ccsbBroadEn_00161 BID gene, encodes complete protein.
KJ905698 - Synthetic construct Homo sapiens clone ccsbBroadEn_15368 BID gene, encodes complete protein.
CU013235 - Homo sapiens BID, mRNA (cDNA clone IMAGE:100000015), complete cds, without stop codon, in Gateway system.
KJ896497 - Synthetic construct Homo sapiens clone ccsbBroadEn_05891 BID gene, encodes complete protein.
AB385066 - Synthetic construct DNA, clone: pF1KB5224, Homo sapiens BID gene for BH3-interacting domain death agonist, complete cds, without stop codon, in Flexi system.
EU446512 - Synthetic construct Homo sapiens clone IMAGE:100070128; IMAGE:100011721; FLH257411.01L BH3 interacting domain death agonist (BID) gene, encodes complete protein.
EU447348 - Homo sapiens Bid precursor, mRNA, partial cds.
CR407603 - Homo sapiens full open reading frame cDNA clone RZPDo834F037D for gene BID, BH3 interacting domain death agonist complete cds, without stopcodon.
CU012947 - Homo sapiens BID, mRNA (cDNA clone IMAGE:100000111), complete cds, with stop codon, in Gateway system.
AK313729 - Homo sapiens cDNA, FLJ94329, Homo sapiens BH3 interacting domain death agonist (BID), mRNA.
LF365625 - JP 2014500723-A/173128: Polycomb-Associated Non-Coding RNAs.
MA601202 - JP 2018138019-A/173128: Polycomb-Associated Non-Coding RNAs.
MG957990 - Homo sapiens BH3 interacting domain death agonist Si6 isoform (BID) mRNA, partial cds.
LF365628 - JP 2014500723-A/173131: Polycomb-Associated Non-Coding RNAs.
MA601205 - JP 2018138019-A/173131: Polycomb-Associated Non-Coding RNAs.
LF365629 - JP 2014500723-A/173132: Polycomb-Associated Non-Coding RNAs.
MA601206 - JP 2018138019-A/173132: Polycomb-Associated Non-Coding RNAs.
JD118864 - Sequence 99888 from Patent EP1572962.
JD129543 - Sequence 110567 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04115 - p53 signaling pathway
hsa04210 - Apoptosis
hsa04650 - Natural killer cell mediated cytotoxicity
hsa05010 - Alzheimer's disease
hsa05014 - Amyotrophic lateral sclerosis (ALS)
hsa05200 - Pathways in cancer
hsa05416 - Viral myocarditis

BioCarta from NCI Cancer Genome Anatomy Project
h_mitochondriaPathway - Role of Mitochondria in Apoptotic Signaling
h_deathPathway - Induction of apoptosis through DR3 and DR4/5 Death Receptors
h_HivnefPathway - HIV-I Nef: negative effector of Fas and TNF
h_chemicalPathway - Apoptotic Signaling in Response to DNA Damage

Reactome (by CSHL, EBI, and GO)

Protein P55957 (Reactome details) participates in the following event(s):

R-HSA-139893 Granzyme-B activates BID by cleavage
R-HSA-139898 Caspase-8 activates BID by cleavage
R-HSA-114352 Sequestration of tBID by BCL-2
R-HSA-168848 tBID binds to inactive BAK protein
R-HSA-139895 tBID activates BAK protein
R-HSA-139897 BAD displaces tBID from BCL-2 sequestration
R-HSA-139920 Translocation of tBID to mitochondria
R-HSA-141367 Myristoylation of tBID by NMT1
R-HSA-168849 tBID binds to inactive BAX protein
R-HSA-139917 tBID activates BAX protein
R-HSA-508162 BH3 only proteins associate with and inactivate anti-apoptotic BCL-XL
R-HSA-75108 Activation, myristolyation of BID and translocation to mitochondria
R-HSA-6803204 TP53 Regulates Transcription of Genes Involved in Cytochrome C Release
R-HSA-111453 BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members
R-HSA-111452 Activation and oligomerization of BAK protein
R-HSA-111447 Activation of BAD and translocation to mitochondria
R-HSA-114294 Activation, translocation and oligomerization of BAX
R-HSA-109606 Intrinsic Pathway for Apoptosis
R-HSA-5633008 TP53 Regulates Transcription of Cell Death Genes
R-HSA-114452 Activation of BH3-only proteins
R-HSA-109581 Apoptosis
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-5357801 Programmed Cell Death
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: BID_HUMAN, NM_001244567, P55957, Q549M7, Q71T04, Q7Z4M9, Q8IY86, uc010grb.1, uc010grb.2
UCSC ID: uc010grb.2
RefSeq Accession: NM_001244567
Protein: P55957 (aka BID_HUMAN)
CCDS: CCDS13748.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.