Human Gene MPST (ENST00000401419.7) Description and Page Index
  Description: Homo sapiens mercaptopyruvate sulfurtransferase (MPST), transcript variant 2, mRNA. (from RefSeq NM_001013436)
RefSeq Summary (NM_001013436): This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: HM005513.1, CX866643.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##
Gencode Transcript: ENST00000401419.7
Gencode Gene: ENSG00000128309.16
Transcript (Including UTRs)
   Position: hg38 chr22:37,019,635-37,029,801 Size: 10,167 Total Exon Count: 3 Strand: +
Coding Region
   Position: hg38 chr22:37,024,216-37,029,514 Size: 5,299 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr22:37,019,635-37,029,801)mRNA (may differ from genome)Protein (297 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
LynxMGIneXtProtOMIMPubMedReactome
Stanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: THTM_HUMAN
DESCRIPTION: RecName: Full=3-mercaptopyruvate sulfurtransferase; Short=MST; EC=2.8.1.2;
FUNCTION: Transfer of a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. May have a role in cyanide degradation or in thiosulfate biosynthesis.
CATALYTIC ACTIVITY: 3-mercaptopyruvate + cyanide = pyruvate + thiocyanate.
SUBUNIT: Monomer or disulfide-linked homodimer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
DOMAIN: The structure consists of 2 domains of very similar conformation, suggesting a common evolutionary origin. However, the sequences of the 2 domains are very different.
SIMILARITY: Contains 2 rhodanese domains.
CAUTION: Was originally (PubMed:1953758) thought to be rhodanese.

-  MalaCards Disease Associations
  MalaCards Gene Search: MPST
Diseases sorted by gene-association score: mercaptolactate-cysteine disulfiduria (41), cyanide poisoning (13), aminoaciduria (5)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 97.39 RPKM in Liver
Total median expression: 1071.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -97.70164-0.596 Picture PostScript Text
3' UTR -114.50287-0.399 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001763 - Rhodanese-like_dom
IPR001307 - Thiosulphate_STrfase_CS

Pfam Domains:
PF00581 - Rhodanese-like domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help

3OLH
- X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P25325
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGD    
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004792 thiosulfate sulfurtransferase activity
GO:0016740 transferase activity
GO:0016784 3-mercaptopyruvate sulfurtransferase activity
GO:0042802 identical protein binding

Biological Process:
GO:0000098 sulfur amino acid catabolic process
GO:0001822 kidney development
GO:0001889 liver development
GO:0009440 cyanate catabolic process
GO:0009636 response to toxic substance
GO:0019346 transsulfuration
GO:0021510 spinal cord development
GO:0070814 hydrogen sulfide biosynthetic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0005829 cytosol
GO:0030054 cell junction
GO:0043005 neuron projection
GO:0045202 synapse
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  KJ904506 - Synthetic construct Homo sapiens clone ccsbBroadEn_13900 MPST gene, encodes complete protein.
HM005513 - Homo sapiens clone HTL-T-200 testicular tissue protein Li 200 mRNA, complete cds.
BC016737 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:24539 IMAGE:4105509), complete cds.
AB208824 - Homo sapiens mRNA for 3-mercaptopyruvate sulfurtransferase variant protein.
AK055733 - Homo sapiens cDNA FLJ31171 fis, clone KIDNE2000046, highly similar to 3-mercaptopyruvate sulfurtransferase.
BC003508 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:10492 IMAGE:3611253), complete cds.
BC009450 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone IMAGE:3531108).
BC018717 - Homo sapiens mercaptopyruvate sulfurtransferase, mRNA (cDNA clone MGC:31798 IMAGE:4131927), complete cds.
X59434 - Human rohu mRNA for rhodanese.
CR456523 - Homo sapiens MPST full length open reading frame (ORF) cDNA clone (cDNA clone C22ORF:pGEM.MPST).
AB463114 - Synthetic construct DNA, clone: pF1KB3110, Homo sapiens MPST gene for mercaptopyruvate sulfurtransferase, without stop codon, in Flexi system.
CU013411 - Homo sapiens MPST, mRNA (cDNA clone IMAGE:100000284), complete cds, without stop codon, in Gateway system.
CU013123 - Homo sapiens MPST, mRNA (cDNA clone IMAGE:100000380), complete cds, with stop codon, in Gateway system.
CR541712 - Homo sapiens full open reading frame cDNA clone RZPDo834B0129D for gene MPST, mercaptopyruvate sulfurtransferase; complete cds, without stopcodon.
BT019636 - Homo sapiens mercaptopyruvate sulfurtransferase mRNA, complete cds.
JD059361 - Sequence 40385 from Patent EP1572962.
JD421282 - Sequence 402306 from Patent EP1572962.
JD053618 - Sequence 34642 from Patent EP1572962.
JD551008 - Sequence 532032 from Patent EP1572962.
JD200166 - Sequence 181190 from Patent EP1572962.
JD477917 - Sequence 458941 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00270 - Cysteine and methionine metabolism
hsa01100 - Metabolic pathways

BioCyc Knowledge Library
PWY-5329 - L-cysteine degradation II

Reactome (by CSHL, EBI, and GO)

Protein P25325 (Reactome details) participates in the following event(s):

R-HSA-9034756 MPST transfers sulfur atom from 3MPYR to form CysS248-MPST
R-HSA-9035484 CysS248-MPST:TXN2 dissociates
R-HSA-9035227 TXN2 binds CysS248-MPST
R-HSA-9012721 MPST transfers sulfur atom from 3MPYR to HSO3- to form S2O3(2-) and PYR
R-HSA-9013471 MPST transfers sulfur from 3MPYR to HCN to form HSCN
R-HSA-9013533 MPST transfers sulfur from sulfanegen to HCN to form HSCN
R-HSA-1614558 Degradation of cysteine and homocysteine
R-HSA-1614635 Sulfur amino acid metabolism
R-HSA-71291 Metabolism of nitrogenous molecules
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: NM_001013436, O75750, P25325, THTM_HUMAN, TST2, uc003aql.1, uc003aql.2, uc003aql.3, uc003aql.4, uc003aql.5
UCSC ID: uc003aql.5
RefSeq Accession: NM_001013436
Protein: P25325 (aka THTM_HUMAN)
CCDS: CCDS13939.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.