Human Gene PIGF (ENST00000306465.8) from GENCODE V41
  Description: Homo sapiens phosphatidylinositol glycan anchor biosynthesis class F (PIGF), transcript variant 2, mRNA. (from RefSeq NM_173074)
RefSeq Summary (NM_173074): This gene encodes a protein involved in glycosylphosphatidylinositol (GPI)-anchor biosynthesis. The GPI-anchor, a glycolipid containing three mannose molecules in its core backbone, is found on many blood cells where it serves to anchor proteins to the cell surface. The encoded protein and another GPI synthesis protein, PIGO, function in the transfer of ethanolaminephosphate to the third mannose in GPI. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Gencode Transcript: ENST00000306465.8
Gencode Gene: ENSG00000151665.13
Transcript (Including UTRs)
   Position: hg38 chr2:46,581,285-46,617,051 Size: 35,767 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg38 chr2:46,588,105-46,615,164 Size: 27,060 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated at UCSC: 2022-05-14 18:57:26

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr2:46,581,285-46,617,051)mRNA (may differ from genome)Protein (206 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSCGAPEnsemblEntrez GeneExonPrimerGencode

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Phosphatidylinositol-glycan biosynthesis class F protein; Short=PIG-F; AltName: Full=GPI11 homolog;
FUNCTION: Involved in GPI-anchor biosynthesis through the transfer of ethanolamine phosphate to the third mannose of GPI (By similarity).
PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol- anchor biosynthesis.
SUBUNIT: Forms a complex with PIGG and PIGO. PIGF is required to stabilize PIGG and PIGO.
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
SIMILARITY: Belongs to the PIGF family.

-  MalaCards Disease Associations
  MalaCards Gene Search: PIGF
Diseases sorted by gene-association score: pre-eclampsia (7), preeclampsia/eclampsia 1 (5), eclampsia (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.33 RPKM in Testis
Total median expression: 235.41 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -34.90103-0.339 Picture PostScript Text
3' UTR -71.00337-0.211 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009580 - GPI_biosynthesis_protein_Pig-F

Pfam Domains:
PF06699 - GPI biosynthesis protein family Pig-F

ModBase Predicted Comparative 3D Structure on Q07326
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
Protein SequenceProtein Sequence    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004307 ethanolaminephosphotransferase activity

Biological Process:
GO:0006506 GPI anchor biosynthetic process
GO:0016254 preassembly of GPI anchor in ER membrane

Cellular Component:
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0016020 membrane
GO:0016021 integral component of membrane

-  Descriptions from all associated GenBank mRNAs
  BC029408 - Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class F, mRNA (cDNA clone MGC:32646 IMAGE:4664848), complete cds.
BC021725 - Homo sapiens phosphatidylinositol glycan anchor biosynthesis, class F, mRNA (cDNA clone MGC:33136 IMAGE:5269689), complete cds.
BC010864 - Homo sapiens cDNA clone IMAGE:3898245, **** WARNING: chimeric clone ****.
D13435 - Homo sapiens PIG-F mRNA for phosphatidyl-inositol-glycan class F, complete cds.
AK291108 - Homo sapiens cDNA FLJ77121 complete cds, highly similar to Homo sapiens phosphatidylinositol glycan, class F (PIGF), transcript variant 1, mRNA.
JD334205 - Sequence 315229 from Patent EP1572962.
CU688644 - Synthetic construct Homo sapiens gateway clone IMAGE:100022132 5' read PIGF mRNA.
CR457000 - Homo sapiens full open reading frame cDNA clone RZPDo834F045D for gene PIGF, phosphatidylinositol glycan, class F; complete cds, incl. stopcodon.
DQ892638 - Synthetic construct clone IMAGE:100005268; FLH188526.01X; RZPDo839D0473D phosphatidylinositol glycan anchor biosynthesis, class F (PIGF) gene, encodes complete protein.
KJ891805 - Synthetic construct Homo sapiens clone ccsbBroadEn_01199 PIGF gene, encodes complete protein.
DQ895876 - Synthetic construct Homo sapiens clone IMAGE:100010336; FLH188522.01L; RZPDo839D0463D phosphatidylinositol glycan anchor biosynthesis, class F (PIGF) gene, encodes complete protein.
JD453470 - Sequence 434494 from Patent EP1572962.
CU692396 - Synthetic construct Homo sapiens gateway clone IMAGE:100023473 5' read PIGF mRNA.
KJ897332 - Synthetic construct Homo sapiens clone ccsbBroadEn_06726 PIGF gene, encodes complete protein.
BC039303 - Homo sapiens cDNA clone IMAGE:4837681, with apparent retained intron.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00563 - Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q07326 (Reactome details) participates in the following event(s):

R-HSA-162742 (ethanolamineP) mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI -> (ethanolamineP) mannose (a1-2) (ethanolamineP) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI
R-HSA-163069 mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI + phosphatidylethanolamine -> (ethanolamineP) mannose (a1-2) mannose (a1-6) (ethanolamineP) mannose (a1-4) glucosaminyl-acyl-PI (acyl-GPI) + diacylglycerol
R-HSA-162710 Synthesis of glycosylphosphatidylinositol (GPI)
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000306465.1, ENST00000306465.2, ENST00000306465.3, ENST00000306465.4, ENST00000306465.5, ENST00000306465.6, ENST00000306465.7, NM_173074, PIGF_HUMAN, Q07326, Q8WW20, uc002rvc.1, uc002rvc.2, uc002rvc.3, uc002rvc.4
UCSC ID: ENST00000306465.8
RefSeq Accession: NM_173074
Protein: Q07326 (aka PIGF_HUMAN)
CCDS: CCDS1828.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.