Human Gene DCLK2 (ENST00000302176.8) Description and Page Index
  Description: Homo sapiens doublecortin like kinase 2 (DCLK2), transcript variant 2, mRNA. (from RefSeq NM_001040261)
RefSeq Summary (NM_001040261): This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Sep 2010].
Gencode Transcript: ENST00000302176.8
Gencode Gene: ENSG00000170390.16
Transcript (Including UTRs)
   Position: hg38 chr4:150,079,028-150,257,438 Size: 178,411 Total Exon Count: 17 Strand: +
Coding Region
   Position: hg38 chr4:150,079,028-150,256,247 Size: 177,220 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsCTDRNA-Seq ExpressionRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:150,079,028-150,257,438)mRNA (may differ from genome)Protein (783 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
HPRDLynxMGIneXtProtOMIMPubMed
Stanford SOURCEUniProtKB

-  Comments and Description Text from UniProtKB
  ID: DCLK2_HUMAN
DESCRIPTION: RecName: Full=Serine/threonine-protein kinase DCLK2; EC=2.7.11.1; AltName: Full=CaMK-like CREB regulatory kinase 2; Short=CL2; Short=CLICK-II; Short=CLICK2; AltName: Full=Doublecortin domain-containing protein 3B; AltName: Full=Doublecortin-like and CAM kinase-like 2; AltName: Full=Doublecortin-like kinase 2;
FUNCTION: Protein kinase with a significantly reduced C(a2+)/CAM affinity and dependence compared to other members of the CaMK family. May play a role in the down-regulation of CRE-dependent gene activation probably by phosphorylation of the CREB coactivator CRTC2/TORC2 and the resulting retention of TORC2 in the cytoplasm (By similarity).
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBUNIT: Binds to and stabilizes microtubules (By similarity). Interacts with MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK9/JNK2, PPP1R9B/NEURABIN-2 and actin (By similarity).
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Colocalizes with microtubules (By similarity).
TISSUE SPECIFICITY: Expressed in the brain, heart and eyes.
DEVELOPMENTAL STAGE: Expressed in fetal brain, and, to a lower extent, in fetal kidney.
DOMAIN: The doublecortin domains are involved in the colocalization with microtubules (By similarity).
PTM: Autophosphorylated (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
SIMILARITY: Contains 2 doublecortin domains.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=BAD92418.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.15 RPKM in Brain - Nucleus accumbens (basal ganglia)
Total median expression: 370.15 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
3' UTR -419.601191-0.352 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020636 - Ca/CaM-dep_Ca-dep_prot_Kinase
IPR003533 - Doublecortin_dom
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF03607 - Doublecortin
PF00069 - Protein kinase domain

ModBase Predicted Comparative 3D Structure on Q8N568
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl   
Protein SequenceProtein Sequence    
AlignmentAlignment    

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0000226 microtubule cytoskeleton organization
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0021766 hippocampus development
GO:0021860 pyramidal neuron development
GO:0030182 neuron differentiation
GO:0035556 intracellular signal transduction
GO:1900181 negative regulation of protein localization to nucleus

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0015630 microtubule cytoskeleton


-  Descriptions from all associated GenBank mRNAs
  LF206157 - JP 2014500723-A/13660: Polycomb-Associated Non-Coding RNAs.
MA441734 - JP 2018138019-A/13660: Polycomb-Associated Non-Coding RNAs.
AB209181 - Homo sapiens mRNA for Hypothetical protein DKFZp761I032 variant protein.
BC032726 - Homo sapiens cDNA clone IMAGE:5532881.
AB384873 - Synthetic construct DNA, clone: pF1KB3903, Homo sapiens DCLK2 gene for serine/threonine-protein kinase DCLK2, complete cds, without stop codon, in Flexi system.
DQ896665 - Synthetic construct Homo sapiens clone IMAGE:100011125; FLH196447.01L; RZPDo839D06155D doublecortin and CaM kinase-like 2 (DCAMKL2) gene, encodes complete protein.
DQ890892 - Synthetic construct clone IMAGE:100003522; FLH166856.01X; RZPDo839E0388D doublecortin and CaM kinase-like 2 (DCAMKL2) gene, encodes complete protein.
DQ893344 - Synthetic construct clone IMAGE:100005974; FLH196451.01X; RZPDo839D06156D doublecortin and CaM kinase-like 2 (DCAMKL2) gene, encodes complete protein.
KJ905028 - Synthetic construct Homo sapiens clone ccsbBroadEn_14422 DCLK2-like gene, encodes complete protein.
KJ905635 - Synthetic construct Homo sapiens clone ccsbBroadEn_15276 DCLK2 gene, encodes complete protein.
BC172430 - Synthetic construct Homo sapiens clone IMAGE:100069124, MGC:199135 doublecortin-like kinase 2 (DCLK2) mRNA, encodes complete protein.
AL834498 - Homo sapiens mRNA; cDNA DKFZp761I032 (from clone DKFZp761I032).
JD068357 - Sequence 49381 from Patent EP1572962.
LF352044 - JP 2014500723-A/159547: Polycomb-Associated Non-Coding RNAs.
MA587621 - JP 2018138019-A/159547: Polycomb-Associated Non-Coding RNAs.
JD475084 - Sequence 456108 from Patent EP1572962.
JD401879 - Sequence 382903 from Patent EP1572962.
JD393726 - Sequence 374750 from Patent EP1572962.
JD057857 - Sequence 38881 from Patent EP1572962.
JD464974 - Sequence 445998 from Patent EP1572962.
JD401172 - Sequence 382196 from Patent EP1572962.
JD541699 - Sequence 522723 from Patent EP1572962.
JD109837 - Sequence 90861 from Patent EP1572962.
JD438775 - Sequence 419799 from Patent EP1572962.
JD166999 - Sequence 148023 from Patent EP1572962.
JD414145 - Sequence 395169 from Patent EP1572962.
JD248707 - Sequence 229731 from Patent EP1572962.
JD364877 - Sequence 345901 from Patent EP1572962.
JD330725 - Sequence 311749 from Patent EP1572962.
BC019831 - Homo sapiens doublecortin-like kinase 2, mRNA (cDNA clone IMAGE:4943185).
JD552027 - Sequence 533051 from Patent EP1572962.
JD391700 - Sequence 372724 from Patent EP1572962.
JD217664 - Sequence 198688 from Patent EP1572962.
JD153429 - Sequence 134453 from Patent EP1572962.
JD307304 - Sequence 288328 from Patent EP1572962.
JD567077 - Sequence 548101 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: C9J5Q9, DCAMKL2, DCDC3B, DCK2, DCLK2_HUMAN, NM_001040261, Q59GC8, Q8N399, Q8N568, uc003ilo.1, uc003ilo.2, uc003ilo.3, uc003ilo.4, uc003ilo.5
UCSC ID: uc003ilo.5
RefSeq Accession: NM_001040261
Protein: Q8N568 (aka DCLK2_HUMAN)
CCDS: CCDS34076.1, CCDS47142.2

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.