Human Gene MGAT5 (ENST00000281923.4) Description and Page Index
  Description: Homo sapiens alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase (MGAT5), transcript variant 2, mRNA. (from RefSeq NM_002410)
RefSeq Summary (NM_002410): The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies. [provided by RefSeq, Oct 2011]. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D17716.1, AF113921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000281923.4/ ENSP00000281923.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##
Gencode Transcript: ENST00000281923.4
Gencode Gene: ENSG00000152127.9
Transcript (Including UTRs)
   Position: hg38 chr2:134,254,072-134,454,621 Size: 200,550 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg38 chr2:134,254,404-134,448,847 Size: 194,444 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsMalaCardsCTDRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesMethods
Data last updated: 2019-09-04

-  Sequence and Links to Tools and Databases
Genomic Sequence (chr2:134,254,072-134,454,621)mRNA (may differ from genome)Protein (741 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaBioGPS
CGAPEnsemblEntrez GeneExonPrimerGeneCardsHGNC
ReactomeStanford SOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
DESCRIPTION: RecName: Full=Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; EC=; AltName: Full=Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase; AltName: Full=GlcNAc-T V; Short=GNT-V; AltName: Full=Mannoside acetylglucosaminyltransferase 5; AltName: Full=N-acetylglucosaminyl-transferase V;
FUNCTION: Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
CATALYTIC ACTIVITY: UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl- beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D- mannosyl-R.
PATHWAY: Protein modification; protein glycosylation.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Single-pass type II membrane protein.
SIMILARITY: Belongs to the glycosyltransferase 18 family.
WEB RESOURCE: Name=GGDB; Note=GlycoGene database; URL="";
WEB RESOURCE: Name=Functional Glycomics Gateway - GTase; Note=Alpha-1,6-mannosylglycoprotein 6-beta-N- acetylglucosaminyltransferaseV; URL="";

-  MalaCards Disease Associations
  MalaCards Gene Search: MGAT5
Diseases sorted by gene-association score: hepatocellular carcinoma (2), bile duct carcinoma (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.50 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 372.16 RPKM

View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -80.60332-0.243 Picture PostScript Text
3' UTR -1985.305774-0.344 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR026116 - GlyclTrfase_18

ModBase Predicted Comparative 3D Structure on Q09328
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
Genome BrowserGenome BrowserNo orthologNo orthologGenome BrowserNo ortholog
Gene Details     
Gene Sorter     
MGIRGDEnsembl WormBase 
Protein SequenceProtein Sequence  Protein Sequence 
AlignmentAlignment  Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0008375 acetylglucosaminyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups
GO:0030144 alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity

Biological Process:
GO:0006486 protein glycosylation
GO:0006487 protein N-linked glycosylation

Cellular Component:
GO:0000139 Golgi membrane
GO:0005794 Golgi apparatus
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0070062 extracellular exosome

-  Descriptions from all associated GenBank mRNAs
  BX647087 - Homo sapiens mRNA; cDNA DKFZp686B24166 (from clone DKFZp686B24166).
E07872 - cDNA encoding fragment of beta-1,N-acetyl glucosaminyl transferase.
D17716 - Homo sapiens mRNA for N-acetylglucosaminyltransferase V, complete cds.
AF113921 - Homo sapiens alpha-1,3(6)-mannosylglycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5) mRNA, complete cds.
BC156313 - Synthetic construct Homo sapiens clone IMAGE:100061790, MGC:190102 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5) mRNA, encodes complete protein.
BC157048 - Synthetic construct Homo sapiens clone IMAGE:100063354, MGC:190758 mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase (MGAT5) mRNA, encodes complete protein.
JD382885 - Sequence 363909 from Patent EP1572962.
JD101629 - Sequence 82653 from Patent EP1572962.
JD327143 - Sequence 308167 from Patent EP1572962.
JD560880 - Sequence 541904 from Patent EP1572962.
JD167805 - Sequence 148829 from Patent EP1572962.
JD208290 - Sequence 189314 from Patent EP1572962.
JD205680 - Sequence 186704 from Patent EP1572962.
JD334645 - Sequence 315669 from Patent EP1572962.
JD324110 - Sequence 305134 from Patent EP1572962.
JD543597 - Sequence 524621 from Patent EP1572962.
JD271477 - Sequence 252501 from Patent EP1572962.
JD336228 - Sequence 317252 from Patent EP1572962.
AK125207 - Homo sapiens cDNA FLJ43217 fis, clone FEBRA2022388.
JD315653 - Sequence 296677 from Patent EP1572962.
JD230514 - Sequence 211538 from Patent EP1572962.
JD418606 - Sequence 399630 from Patent EP1572962.
JD182979 - Sequence 164003 from Patent EP1572962.
JD263620 - Sequence 244644 from Patent EP1572962.
JD503741 - Sequence 484765 from Patent EP1572962.
JD137324 - Sequence 118348 from Patent EP1572962.
JD126343 - Sequence 107367 from Patent EP1572962.
JD335190 - Sequence 316214 from Patent EP1572962.
JD314408 - Sequence 295432 from Patent EP1572962.
JD375674 - Sequence 356698 from Patent EP1572962.
AK056461 - Homo sapiens cDNA FLJ31899 fis, clone NT2RP7004173.
JD144811 - Sequence 125835 from Patent EP1572962.
JD178523 - Sequence 159547 from Patent EP1572962.
JD042438 - Sequence 23462 from Patent EP1572962.
JD466883 - Sequence 447907 from Patent EP1572962.
JD101601 - Sequence 82625 from Patent EP1572962.
JD178944 - Sequence 159968 from Patent EP1572962.
JD335539 - Sequence 316563 from Patent EP1572962.
DQ601732 - Homo sapiens piRNA piR-39798, complete sequence.
JD283627 - Sequence 264651 from Patent EP1572962.
JD549366 - Sequence 530390 from Patent EP1572962.
JD358242 - Sequence 339266 from Patent EP1572962.
AF055029 - Homo sapiens clone 24711 mRNA sequence.
JD299598 - Sequence 280622 from Patent EP1572962.
JD172121 - Sequence 153145 from Patent EP1572962.
JD235818 - Sequence 216842 from Patent EP1572962.
JD054354 - Sequence 35378 from Patent EP1572962.
JD490435 - Sequence 471459 from Patent EP1572962.
BC041917 - Homo sapiens hypothetical protein LOC151162, mRNA (cDNA clone IMAGE:5300004).
JD226805 - Sequence 207829 from Patent EP1572962.
JD426438 - Sequence 407462 from Patent EP1572962.
JD191490 - Sequence 172514 from Patent EP1572962.
JD418188 - Sequence 399212 from Patent EP1572962.
JD490326 - Sequence 471350 from Patent EP1572962.
JD236359 - Sequence 217383 from Patent EP1572962.
JD521384 - Sequence 502408 from Patent EP1572962.
JD268709 - Sequence 249733 from Patent EP1572962.
JD382891 - Sequence 363915 from Patent EP1572962.
JD236753 - Sequence 217777 from Patent EP1572962.
JD416531 - Sequence 397555 from Patent EP1572962.
JD126664 - Sequence 107688 from Patent EP1572962.
JD353984 - Sequence 335008 from Patent EP1572962.
JD116015 - Sequence 97039 from Patent EP1572962.
JD086710 - Sequence 67734 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa00510 - N-Glycan biosynthesis
hsa01100 - Metabolic pathways

Reactome (by CSHL, EBI, and GO)

Protein Q09328 (Reactome details) participates in the following event(s):

R-HSA-975916 Addition of GlcNAc to position 5 by MGAT5
R-HSA-975577 N-Glycan antennae elongation
R-HSA-975576 N-glycan antennae elongation in the medial/trans-Golgi
R-HSA-948021 Transport to the Golgi and subsequent modification
R-HSA-446203 Asparagine N-linked glycosylation
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: D3DP70, GGNT5, MGT5A_HUMAN, NM_002410, Q09328, uc002ttw.1, uc002ttw.2, uc002ttw.3, uc002ttw.4, uc002ttw.5, uc002ttw.6
UCSC ID: uc002ttw.6
RefSeq Accession: NM_002410
Protein: Q09328 (aka MGT5A_HUMAN)
CCDS: CCDS2171.1

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.