Human Gene NUF2 (ENST00000271452.8) Description and Page Index
Description: Homo sapiens NUF2 component of NDC80 kinetochore complex (NUF2), transcript variant 1, mRNA. (from RefSeq NM_145697) RefSeq Summary (NM_145697): This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008]. Gencode Transcript: ENST00000271452.8 Gencode Gene: ENSG00000143228.13 Transcript (Including UTRs) Position: hg38 chr1:163,321,954-163,355,759 Size: 33,806 Total Exon Count: 14 Strand: + Coding Region Position: hg38 chr1:163,326,052-163,355,469 Size: 29,418 Coding Exon Count: 13
ID:NUF2_HUMAN DESCRIPTION: RecName: Full=Kinetochore protein Nuf2; Short=hNuf2; Short=hNuf2R; Short=hsNuf2; AltName: Full=Cell division cycle-associated protein 1; FUNCTION: Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. SUBUNIT: Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24- SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C- terminal regions of both subunits of the NDC80/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B. Directly interacts with CENPE; this interaction determines CENPE kinetochore localization. INTERACTION: Q02224:CENPE; NbExp=9; IntAct=EBI-724102, EBI-1375040; O14777:NDC80; NbExp=10; IntAct=EBI-724102, EBI-715849; SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. NDC80 is required for efficient kinetochore localization. PTM: Can be phosphorylated by AURKA and AURKB. SIMILARITY: Belongs to the NUF2 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9BZD4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.