Description: Homo sapiens PDZ binding kinase (PBK), mRNA. RefSeq Summary (NM_018492): This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]. Transcript (Including UTRs) Position: hg19 chr8:27,667,138-27,695,349 Size: 28,212 Total Exon Count: 8 Strand: - Coding Region Position: hg19 chr8:27,667,822-27,690,630 Size: 22,809 Coding Exon Count: 7
ID:TOPK_HUMAN DESCRIPTION: RecName: Full=Lymphokine-activated killer T-cell-originated protein kinase; EC=2.7.12.2; AltName: Full=Cancer/testis antigen 84; Short=CT84; AltName: Full=MAPKK-like protein kinase; AltName: Full=Nori-3; AltName: Full=PDZ-binding kinase; AltName: Full=Spermatogenesis-related protein kinase; Short=SPK; AltName: Full=T-LAK cell-originated protein kinase; FUNCTION: Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. ENZYME REGULATION: Activated by phosphorylation (By similarity). SUBUNIT: Interacts with DLG1 and TP53. INTERACTION: P10398:ARAF; NbExp=3; IntAct=EBI-536853, EBI-365961; P04637:TP53; NbExp=7; IntAct=EBI-536853, EBI-366083; TISSUE SPECIFICITY: Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules. PTM: Phosphorylated; in a cell-cycle dependent manner at mitosis. SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase kinase subfamily. SIMILARITY: Contains 1 protein kinase domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00069 - Protein kinase domain PF07714 - Protein tyrosine kinase
SCOP Domains: 56112 - Protein kinase-like (PK-like)
ModBase Predicted Comparative 3D Structure on Q96KB5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000278 mitotic cell cycle GO:0001933 negative regulation of protein phosphorylation GO:0006468 protein phosphorylation GO:0016310 phosphorylation GO:0032435 negative regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032873 negative regulation of stress-activated MAPK cascade GO:0034644 cellular response to UV GO:0050728 negative regulation of inflammatory response
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
